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What is Myco-Substitutes?

Myco-Substitutes is a startup company that specializes in waste and biomass valorization. It was formed by a team of scientist and engineers. It has its headquarters in Tamale the capital city of the Northern region of Ghana. It also operates in Tarkwa where some of its members are based.

About the Technology

ROFSIEQ technology developed by Myco-Substitutes uses faecal sludge as a medium for producing mycelia that are converted into valuable products including boots, aprons, and gloves for sanitation workers, toilet wall designs, frames, artefacts and more. Biochar and briquettes are additional products the technology offers. ROFSIEQ is an eco-friendly sewage treatment that uses viruses and fungi to treat and feed on faecal waste and produce yarns and a leather substitute. Ghanaian environmental technologist Abubakari Zarouk Imoro led the creation of Myco-Substitutes in response to the health risks of informal toilets and dumping of faecal sludge. The treatment process uses viruses known as bacteriophages to feed on and remove bacteria from faecal sludge. Filamentous fungi, such as Basidiomycetes and Ascomycetes (Rhizopus and Aspergillus spp.), are introduced to the remaining solid waste, which functions as a growth medium to cultivate mycelia, the root-like structure of the fungi. Toilet paper in the sludge functions as a carbon source for mycelial growth. After 72 to 120 hours, 10 litres of sludge can produce 500 grams of mycelia for harvest. Mycelia are used to create an alternative to cotton or synthetic thread for the garment industry. The leather-like fungal mycelia (an alternative to animal hide) is grown in 500 mm x 500 mm sheets for tanning and making bags, belts, and shoes. The strength of cowhide can be achieved by combining several sheets of mycelial leather. The threads are straightened and combined using natural latex and fibre from the Calotropis procera (giant milkweed) and cotton from the Kapok tree when additional strength is required. The mycelia in combination with the giant milkweed fibre are spun into a yarn with a mass similar to cotton. The Myco-Substitute’s technology, which is in pre-pilot phase, produces between one and five balls of yarn a day. This is dependent on factors including the fungal species used, available space, and the availability of giant milkweed. Imoro and his team receive faecal sludge (faeces, toilet paper and black water) from wastewater treatment plants for treatment and processing into the growth medium for mycelia cultivation. They analyse it for bacteriophages, which are isolated and cultivated over 72 hours before being added back to the sludge where they kill bacteria present. The liquid is then treated with chlorine or UV light and can be used for irrigation. The remaining sludge is converted into biochar, which can enrich soil or create environmentally friendly briquettes. Imoro and his team have created a circular economy for faecal sludge, where value is created from all parts of the waste. “By generating value from sustainable recycling of faecal sludge, we are creating the incentive for people to responsibly dispose of their waste. We’ve also created an affordable alternative to cotton and leather imports in the textile industry.”  Zarouk Abubakari Imoro

Transforming Faecal Sludge into Sustainable Resources
Myco-Substitutes developed the Reuse of Faecal Sludge for Income and Environmental Quality Management (ROFSIEQ) technology to valorize faecal sludge. The ROFSIEQ technology treats faecal sludge with bacteriophages and then converts the treated sludge into suitable media for the production of fungal mycelia. The fungal mycelia are then used to produce fabrics for clothes and leather for shoes, and bags. The spent growth media (treated faecal sludge) after mycelia harvesting is converted into biochar for soil quality improvement and briquettes as a source of fuel. Our proposed solution maximizes the use of resources (land space, water and virgin materials). And unlike earlier interventions such as anaerobic digestion, composting and use of stabilization ponds, our initiative relatively does not need large space to establish and does not leave behind waste that requires further treatment or that may lead to secondary pollution (ROFSIEQ is a zero-waste technology).
Economic and Environmental Impact
In line with the sustainable development goal one (no poverty), the technology has the potential to provide jobs to the poor when scaled up and thus will enhance local economies. It also addresses the sustainable development goal six (water and sanitation for all) leading to goal three (good health and wellbeing). It provides an alternative source of leather to animal leather, which consumes a considerable amount of water (104,310 L per hide ~1 m2) (Mekonnen and Heokstra, 2010) to process. In addition, the technology will contribute to the reduction in pollution resulting from the poor management of faecal sludge, the release of methane gas from wastewater treatment plants and minimize land degradation (Goal 13: Combat climate change and its impacts) associated with the use of fuel wood/charcoal. The ROFSIEQ initiative will create a huge impact on local businesses aside the sanitation solution it will provide at scale. The local smock industry has complained about the inadequacy of threads for smock production (Abdul-Rahim et al., 2016) and in some cases, high cost of threads required to produce durable smocks. This has affected the sustainability and scaling of a number of startups in the smock and Kente industries. With the intervention of ROFSIEQ, affordable mycelia-based threads will be produced to service these industries. Local shoe and bag makers will also have affordable leather to work with. The initiative can also help minimize the quantity of trees fell to produce charcoal through the production of briquettes from charred faecal sludge.